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A major goal of microfluidics is to mimic bench-level fluid manipulations within the droplet format in order to take advantage of smaller reaction volumes, massively-parallel scalability, compartmentalization and decreased reagent use. The ability to generate droplets at kHz frequency permits massively parallel reaction schemes with precision control of reaction volumes due to the highly monodisperse nature of microfluidic droplet generation.
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Droplet-based sample partitioning into miniscule volumes allows for faster reaction kinetics due to decreased diffusion times compared to bulk scale reactions. Most commonly, aqueous fluids are encapsulated into droplets surrounded by a continuous phase of an oil surfactant mixture.
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This method can be performed in a serial array to obtain an arbitrary degree of wash efficiency and integrated into lab-on-a-chip systems for use in multi-step microfluidic bioassays or single-cell genomic applications requiring high-fidelity washing steps within droplets.ĭroplet microfluidics involves sample partitioning into nanoliter or smaller volumes and has been extensively used in a variety of fields including biology, medicine, chemistry and physics.
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Using a two-stage device, combined microparticle retention rates (up to 97.5%) and high wash efficiency (92.9%) is demonstrated using dye absorbance and fluorescence. The device can operate at high throughput (50 Hz) while preserving input droplet volume at the collection outlet as verified using high speed imaging. Here we present a microfluidic device architecture that utilizes wash buffer injection preceding a splitting junction in proximity to a magnetic field to transfer paramagnetic microparticles across a concentration gradient within a single droplet. washing) at high-throughput is challenging to implement. Droplet microfluidic operations such as droplet generation, sorting, and fluid addition are well established however, fluid exchange (i.e.
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Utilizing a 12-bit digital CMOS sensor with 1,024 x 1,024 pixel resolution, the FASTCAM MiniĪX delivers up to 4,000 fps at full resolution and up to 540,000 fps at reduced resolution.įollowing are some sample frame rates and resolutions for the Mini AX100 and Mini AX50.Droplet microfluidics has emerged as a promising technique to perform high-throughput, massively-parallel chemical and molecular biological reactions.
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Using the ISO 12232 Ssat standard, the Mini AX is rated at ISO 40,000 monochrome and ISO 16,000 color! Frame rate and resolution In fact, with the exception of image intensified products, the Mini AX has better light sensitivity than any other high-speed camera ever offered by Photron. The FASTCAM Mini AX provides extraordinary light sensitivity. The Mini AX200 will provide 6,400 frames per second at full resolution. Note that in a late September 2015 timeframe, a third model – the FASTCAM Mini AX200 – will also be available. The Mini AX50 is the lower priced camera, providing 2,000 fps at full resolution. The Mini AX100 is the faster camera, providing over 4,000 frames per second at full resolution. The FASTCAM Mini AX is currently available in two models that differ only in frame rate erformance. The Mini AX is well-suited to many different applications such as general research, offboard automotive testing, fluid dynamics, material sciences, ballistics, explosives testing and more. The megapixel Mini AX provides a combination of high frame rates, extraordinary light sensitivity and outstanding image quality that is superior to any camera in its class. Photron is pleased to announce the newest member of the “Mini” family of small and lightweight high-speed cameras - the FASTCAM Mini AX.
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Photron, FASTCAM Mini AX teaser movie Overview Photron FASTCAM Mini AX100 and Mini AX50.